Time Kill Charts

TIME KILL CHART for Anasept® Antimicrobial Skin & Wound Cleanser & Anasept® Antimicrobial Wound Irrigation Solution

  Table of In Vitro Microbial Activity
Test Organisms: Initial Microorganism Count/ML                     Exposure time / % Kill
 Pathogenic Bacteria: 30 seconds 1 minute 5 minutes
Acinetobacter baumannii                     107         – 99.089% 99.98%
Carbapenem Resistant Escherichia coli (CRE)                     106  99.999%  99.999%  99.999%
Clostridium difficile                     105 100% 100% 100%
Escherichia coli                     107 100% 100% 100%
Methicillin Resistant Staphylococcus aureus (MRSA)                     107 100% 100% 100%
Proteus mirabilis                     108 99.998% 100% 100%
Pseudomonas aeruginosa                     107 100% 100% 100%
Serratia marcescens                     107  100% 100% 100%
Staphylococcus aureus                     107 100% 100% 100%
Vancomycin Resistant Enterococcus faecalis (VRE)                     107 100% 100% 100%
Pathogenic Fungi:
Aspergillus niger                     107 99.99% 99.9999% 100%
Candida albicans                     107 99.1% 99.9% 100%
Cadida auris                    105 99.1% >99.9% >99.9%

 

  Table of Sporicidal Activity
Test Spore:                 Initial Spore Count/ML Exposure time Percent Reduction Log Reduction
Clostridium difficile- spore                     106   15 minutes 99.999% >5.7

 

  Table of Virucidal Activity
Test Virus: Initial Virus Count/ML Exposure time Percent Reduction Log Reduction
HIV-Type 1 (Human Immuno Deficiency Virus)  106   5 minutes 99.997% 4.5

 

 


TIME KILL CHART for Anasept® Antimicrobial Skin & Wound Gel Formula

  Table of In Vitro Microbial Activity
Test Organisms: Initial Organism Count                        Exposure time / % Kill
 Pathogenic Bacteria: 1 minute 3 minutes 5 minutes 10 Minutes
Acinetobacter baumannii               107 98.56% 99.99% 99.998% 99.9999%
Carbapenem Resistant E. coli (CRE)               106 99.999% 99.999% 99.999% 99.999%
Clostridium difficile               105 100% 100% 100% 100%
Escherichia coli               107 99.25% 99.986% 99.9995% 100%
Methicillin Resistant Staphylococcus aureus (MRSA)               107 100% 100% 100% 100%
Proteus mirabilis               107 99.888% 99.998% 99.9998% 100%
Pseudomonas aeruginosa               107 99.996% 100% 100% 100%
Serratia marcescens               107 100% 100% 100% 100%
Staphylococcus aureus               107 100% 100% 100% 100%
Vancomycin Resistant Enterococcus faecalis (VRE)               107 100% 100% 100% 100%
Pathogenic Fungi:
Aspergillus niger               106 100% 100% 100% 100%
Candida albicans               106 100% 100% 100% 100%
Candida auris               105 >99.9% >99.9% >99.9% N/A

 

  Table of Sporicidal Activity
Test Spore:                 Initial Spore Count/ML Exposure time Percent Reduction Log Reduction
Clostridium difficile- spore                     106   15 minutes 99.99% >4.0

 

  Table of Virucidal Activity
Test Virus: Initial Virus Count/ML Exposure time Percent Reduction Log Reduction
HIV-Type 1 (Human Immuno Deficiency Virus)  106   5 minutes 99.97% 3.5

 


TIME KILL STUDIES – 24 HOUR CHALLENGE Table of Antimicrobial Activity

Test Organisms: Initial Organism Ct. / Re-challenge Organism Ct.
 
Exposure time after re-challenge at 24 hours/% Kill
 Pathogenic Bacteria: 5 minutes 10 minutes 15 minutes
Acinetobacter baumannii 107  / 107    13.64% 85.27% 99.25%
Escherichia coli 107  / 107    71.25% 96.63% 99.49%
Methicillin Resistant Staphylococcus aureus (MRSA) 107  / 107    95.69% 99.38% 99.78%
Proteus mirabilis 107  / 107    67.14% 97.71% 99.74%
Pseudomonas aeruginosa 107  / 107    84.35% 98% 99.88%
Serratia marcescens 107  / 107    96% 99.36% 99.94%
Staphylococcus aureus 107  / 107    95.91% 96.45% 99.16%
Vancomycin Resistant Enterococcus faecalis (VRE) 107  / 107    92.8% 96.9% 99.61%
Pathogenic Fungi:
Candida albicans 106  / 106 98.89% 99.99% 99.9996%
Mix of all above including Candida albicans 107  / 107    88.75% 97.31% 99.8%

TIME KILL CHART for Silver-Sept® Antimicrobial Skin & Wound Gel Formula

Pathogenic Bacteria: Initial Microorganism Count                          Exposure time / % Kill
1 hour 2 hours 1 day
Escherichia coli                 107 99.99985% 100% 100%
Methicillin Resistant Staphylococcus aureus (MRSA)                 107 98.32% 100% 100%
Proteus mirabilis                 107 99.9998% 100% 100%
Pseudomonas aeruginosa                 107 99.9996% 100% 100%
Serratia marcescens                 107 99.9538% 100% 100%
Staphylococcus aureus                 107 99.77% 100% 100%
Vancomycin Resistant Enterococcus faecalis (VRE)                 107 98.27% 100% 100%
Pathogenic Fungi:
Aspergillus niger                 106 99.99% 99.9999% 100%
Candida albicans                 106 99.1% 99.9% 100%