Time Kill Charts

Please note: In the time kill studies below, extremely high concentrations of pathogenic microorganisms were exposed to Anasept over the course of precisely timed intervals in the presence of an interfering substance that simulated the organic load condition of the wound environment and is known to inhibit the action of antimicrobial agents.

 


TIME KILL CHART for Anasept® Antimicrobial Skin & Wound Cleanser & Anasept® Antimicrobial Wound Irrigation Solution

  Table of In Vitro Microbial Activity
Test Organisms: Initial Microorganism Count/ML                     Exposure time / % Kill
 Pathogenic Bacteria: 30 seconds 1 minute 5 minutes
Acinetobacter baumannii                     107          –  99.089%  99.98%
Carbapenem Resistant Escherichia coli (CRE)                     106  99.999%  99.999%  99.999%
Clostridium difficile                     105 100% 100% 100%
Escherichia coli                     107 100% 100% 100%
Methicillin Resistant Staphylococcus aureus (MRSA)                     107 100% 100% 100%
Proteus mirabilis                     108 99.998% 100% 100%
Pseudomonas aeruginosa                     107 100% 100% 100%
Serratia marcescens                     107  100% 100% 100%
Staphylococcus aureus                     107 100% 100% 100%
Vancomycin Resistant Enterococcus faecalis (VRE)                     107 100% 100% 100%
Pathogenic Fungi:
Aspergillus niger                     107 99.99% 99.9999% 100%
Candida albicans                     107 99.1% 99.9% 100%
Cadida auris                    105 99.1% >99.9% >99.9%

 

  Table of Sporicidal Activity
Test Spore:                 Initial Spore Count/ML Exposure time Percent Reduction Log Reduction
Clostridium difficile- spore                     106   15 minutes 99.999% >5.7

 

  Table of Virucidal Activity

 

Test Virus: Initial Virus Count/ML Exposure time Percent Reduction Log Reduction
HIV-Type 1 (Human Immuno Deficiency Virus) 106 5 minutes 99.997% 4.5
SARS Related Coronavirus 2 105.25 5 minutes 99.98% 3.75
Human Coronavirus Strain 229E 105.5 5 minutes 99.99% 4.0

 

 


TIME KILL CHART for Anasept® Antimicrobial Skin & Wound Gel Formula

  Table of In Vitro Microbial Activity
Test Organisms: Initial Organism Count                        Exposure time / % Kill
 Pathogenic Bacteria: 1 minute 3 minutes 5 minutes 10 Minutes
Acinetobacter baumannii               107 98.56% 99.99% 99.998% 99.9999%
Carbapenem Resistant E. coli (CRE)               106 99.999% 99.999% 99.999% 99.999%
Clostridium difficile               105 100% 100% 100% 100%
Escherichia coli               107 99.25% 99.986% 99.9995% 100%
Methicillin Resistant Staphylococcus aureus (MRSA)               107 100% 100% 100% 100%
Proteus mirabilis               107 99.888% 99.998% 99.9998% 100%
Pseudomonas aeruginosa               107 99.996% 100% 100% 100%
Serratia marcescens               107 100% 100% 100% 100%
Staphylococcus aureus               107 100% 100% 100% 100%
Vancomycin Resistant Enterococcus faecalis (VRE)               107 100% 100% 100% 100%
Pathogenic Fungi:
Aspergillus niger               106 100% 100% 100% 100%
Candida albicans               106 100% 100% 100% 100%
Candida auris               105 >99.9% >99.9% >99.9% N/A

 

  Table of Sporicidal Activity
Test Spore:                 Initial Spore Count/ML Exposure time Percent Reduction Log Reduction
Clostridium difficile- spore                     106   15 minutes 99.99% >4.0

 

  Table of Virucidal Activity
Test Virus: Initial Virus Count/ML Exposure time Percent Reduction Log Reduction
HIV-Type 1 (Human Immuno Deficiency Virus) 106   5 minutes 99.97% 3.5
SARS Related Coronavirus 2 105.25 5 minutes 99.98% 3.75
Human Coronavirus Strain 229E 105.5 5 minutes 99.9% 3.0

 


TIME KILL STUDIES – 24 HOUR CHALLENGE Table of Antimicrobial Activity

Test Organisms:       Initial Organism Ct. /       Re-challenge Organism Ct.
 
Exposure time after re-challenge at 24 hours/% Kill
 Pathogenic Bacteria: 5 minutes 10 minutes 15 minutes
Acinetobacter baumannii 107  / 107    13.64% 85.27% 99.25%
Escherichia coli 107  / 107    71.25% 96.63% 99.49%
Methicillin Resistant Staphylococcus aureus (MRSA) 107  / 107    95.69% 99.38% 99.78%
Proteus mirabilis 107  / 107    67.14% 97.71% 99.74%
Pseudomonas aeruginosa 107  / 107    84.35% 98% 99.88%
Serratia marcescens 107  / 107    96% 99.36% 99.94%
Staphylococcus aureus 107  / 107    95.91% 96.45% 99.16%
Vancomycin Resistant Enterococcus faecalis (VRE) 107  / 107    92.8% 96.9% 99.61%
Pathogenic Fungi:
Candida albicans 106  / 106 98.89% 99.99% 99.9996%
Mix of all above including Candida albicans 107  / 107    88.75% 97.31% 99.8%


TIME KILL CHART for Silver-Sept® Antimicrobial Skin & Wound Gel Formula

Pathogenic Bacteria: Initial Microorganism Count                          Exposure time / % Kill
1 hour 2 hours 1 day
Escherichia coli                 107 99.99985% 100% 100%
Methicillin Resistant Staphylococcus aureus (MRSA)                 107 98.32% 100% 100%
Proteus mirabilis                 107 99.9998% 100% 100%
Pseudomonas aeruginosa                 107 99.9996% 100% 100%
Serratia marcescens                 107 99.9538% 100% 100%
Staphylococcus aureus                 107 99.77% 100% 100%
Vancomycin Resistant Enterococcus faecalis (VRE)                 107 98.27% 100% 100%
Pathogenic Fungi:
Aspergillus niger                 106 99.99% 99.9999% 100%
Candida albicans                 106 99.1% 99.9% 100%